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. Author manuscript; available in PMC: 2013 Jun 16.
Published in final edited form as: Nat Protoc. 2009 Jul 23;4(8):1192–1201. doi: 10.1038/nprot.2009.100

Figure 1.

Figure 1

Figure 1

Schematic diagram of the DF and TF lentiviral constructs as well as the underlying mechanism of each imaging modality. (a) The DF construct contains enhanced green fluorescent protein (eGFP) and firefly luciferase (Fluc) reporter genes linked by 5 amino acid linker (GSHGD). The TF construct contains monomeric red fluorescent protein (mRFP), Fluc, and herpes simplex virus thymidine kinase (HSVtk) reporter genes, with the 3 fusion proteins joined by a 14-amino acid (LENSHASAGYQAST) and 8-amino acid (TAGPGSAT) linker, respectively. (b) Diagram illustrating the mechanism of each imaging modality based on their respective reporter genes using the TF construct as an example.