FIG. 2.

Chromosome structures of the host (TM1-1) (A), the single-crossover integrant (B), and the double-crossover integrant (C). Integrants were obtained after cultivation of a transformant harboring the pSintA1 plasmid on LM17 at 37°C. The chromosome structures were estimated by the PCR experiment data shown in Table 4. The phenotype of each clone is shown in an open square. Relevant PCR primers used in this study are shown.