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Comparisons of transformation efficiencies using thyA and ermA
| Plasmid | Selection marker | Selection mediuma | No. of transformants/ μg of plasmid DNA |
|---|---|---|---|
| pSYTLb | thyALb | LM17 | 6.4 × 104 |
| ermA | LM17 + Em + Thy | 6.2 × 104 | |
| pXTLb | thyALb | LM17 | 2.8 × 102 |
| ermA | LM17 + Em + Thy | 3.0 × 102 | |
| pX4041 | thyASt | LM17 | 2.0 × 102 |
| ermA | LM17 + Em + Thy | 2.3 × 102 |
Erythromycin (Em) and thymidine (Thy) were added at 25 and 20 μg/ml, respectively.
