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. Author manuscript; available in PMC: 2014 Apr 25.
Published in final edited form as: Cell Rep. 2013 Apr 11;3(4):988–995. doi: 10.1016/j.celrep.2013.03.015

Figure 4. The distribution of mRNAs at the NE and synaptic sites in disrupted in torsin mutants.

Figure 4

(Also see Fig. SF4).

(A,B) FISH to body wall muscles showing the nuclear distribution of (A) par6 and (B) magi transcripts in wild type and torsin mutants.

(C) Quantification of FISH signal outside the nucleus. N [nuclei;larvae]= [18;6],[18;6],[18;6],[14;6].

(D, E) FISH to body wall muscles showing the distribution of (D) par6 and (E) magi transcript at the NMJ in wild type and torsin null mutants.

(F, G) Distribution of (F) Par6 and (G) Magi immunoreactivity at the NMJ in wild type and torsin mutants.

(H, I) Quantification of postsynaptic (H) Par6 and (I) Magi immunoreactive signal, normalized to wild type control. N([NMJs;larvae])= [16;6],[18;6] for H and [17;6],[15;6] for I.

(J) NMJs in wild type and torsin mutants labeled with anti-HRP and anti-DLG showing reduced size and increased ghost boutons (arrowheads) in torsin mutants.

(K, L) Quantification of the number of (K) synaptic boutons and (L) ghost boutons. N([NMJs;larvae])= [19;10],[18;10],[19;10],[19;10],[20;10],[19;10],[19;10] for K,L. Error bars represent ±SEM (**= p<0.001, ***= p<0.0001).

Calibration scale (μm)= A,B:3; D–G:10; J:20.