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. 2013 Feb 27;57(1):e10. doi: 10.4081/ejh.2013.e10

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©Copyright S. Tetè et al., 2013

This work is licensed under a Creative Commons Attribution NonCommercial 3.0 License (CC BY-NC 3.0).

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A) Immunohistochemical analysis of BSP expression in Group 1 and Group 2 specimens, respectively. Weak BSP immunolabeling in Group 1 and moderate BSP immunolabeling in Group 2 bone tissue; no BSP immunostaining is seen in negative control. B) Immunohistochemical analysis of SPARC expression, in Group 1 and Group 2 specimens, respectively. Magnification 20×. Group 1: bone tissue specimenss obtained from equinederived bone substitute grafted area; Group 2: bone tissue specimens obtained from calvaria bone grafted area; C(-), negative control. Weak SPARC immunolabeling in Group 1 and moderate SPARC immunolabeling in Group 2 bone tissue; no SPARC immunostaining is seen in negative control. C) Graphic representation of densitometric analysis of BSP and SPARC positive area ± SD determined by direct visual counting of ten fields (mean values) for each of five slides per specimens at 20× magnification (*P<0.001; **P<0.001).