a, qRT-PCR of PVT1-encoded microRNAs confirms up-regulation of miR-1204 in PVT1-MYC fusion(+) Group 3s: MYC-balanced/fusion(−), n=4; MYC-amplified/fusion(−), n=6; MYC-amplified/fusion(+), n=8. Error bars represent standard error of the mean (SEM) and reflect variability among samples. b, c Knockdown of miR-1204 attenuates the proliferative capacity of PVT1-MYC fusion(+) MED8A medulloblastoma cells (b) but has no effect on fusion(−) ONS76 cells (c). Error bars represent the standard deviation (SD) of triplicate experiments.