Table 3.

List of Contigs differentially expressed.

Contig	Annotation	Best hit	E-value	Shallow library (−5 m)	Deep library (−25 m)	Regulation	RT-qPCR regulation
Pooc_B_c42	N(2),N(2)-dimethylguanosine tRNA methyltransferase	Q34941	3.0e-14	15	0	UP-5 m	UP-5 m
Pooc_B_c444	F-box protein At5g67140	Q9FH99	2.0e-28	16	0	UP-5 m
Pooc_B_c209	no hit	–	–	15	0	UP-5 m
Pooc_B_c205	no hit	–	–	341	19	UP-5 m
Pooc_B_c18	no hit	–	–	38	0	UP-5 m
Pooc_B_c217	cellular_component	GO:0005575	–	0	17	UP-25 m	UP-25 m

List of Contigs showing significant EST frequency difference between libraries (p ≤ 0.05). For each Contig, name, annotation, best hits E-value, number of EST reads in each library, and regulation signals are indicated. Differential expression between the two depths reported in this work (shallow −5 m, deep −25 m) was tested in RT-qPCR experiment by Serra et al. (2012b). RT-qPCR results are also reported in Figure S1 for Contigs Pooc_B_c42 and Pooc_B_c217 (in bold).