TABLE 5.

Detection and quantification limits of RTi-PCR assays with suspensions of standard curve strains L. monocytogenes UdG 1010 and L. innocua strain CECT 910^a

Species	Gene	Slope	R2	Mean ± SD CFU in the reaction (3 replicates)	Signal ratio (positive signals/9 reactions)	CT values
						Mean	SD
L. monocytogenes	hly	−3.81	0.998	291,250 ± 13,150	9	18.01	0.11
				29,125 ± 1,315.0	9	21.33	0.08
				2,912 ± 131.50	9	25.00	0.08
				291 ± 13.15	9	29.33	0.10
				29 ± 1.31	9	33.10	0.39
				3 ± 0.13	8	37.54	4.71
	hly (AmpliFluor)	−3.84	0.996	291,250 ± 13,150	9	27.54	0.09
				29,125 ± 1,315.0	9	30.85	0.30
				2,912 ± 131.50	9	35.38	0.36
				291 ± 13.15	9	38.83	0.34
				29 ± 1.31	2	44.30	1.40
				3 ± 0.13	0	45.00	0.00
	iap	−3.71	0.996	291,250 ± 13,150	9	17.72	0.19
				29,125 ± 1,315.0	9	20.91	0.32
				2,912 ± 131.50	9	24.24	0.23
				291 ± 13.15	9	28.84	0.12
				29 ± 1.31	9	32.29	0.46
				3 ± 0.13	6	40.20	7.40
L. innocua	lin02483	−3.85	0.997	231,661 ± 13,714	9	17.75	0.14
				23,166 ± 1,371.4	9	20.93	0.17
				2,317 ± 137.14	9	24.71	0.23
				232 ± 13.71	9	29.21	0.25
				23 ± 1.38	9	32.95	0.48
				2 ± 0.14	5	42.81	6.88

^aRegression curves were calculated from approximately 3 × 10⁵ to 30 template molecules.