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Vinculin-null cells engineered to express vinculin variants. (A) Vinculin variants fused to eGFP: WT, head domain (VH), auto-inhibition mutant (T12), talin-binding mutant (A50I), and tail domain (VT). (B) Western blot analysis of engineered cell lines confirmed expression of vinculin constructs. Vinculin expression was repressed in presence of anhydrotetracycline (aTc, 100 ng/mL). (C) eGFP-vinculin localized to FAs for both MEF1- and MEF2-derived lines. (Scale bar, 10 µm.)
