Fig. 1.

Tomato SlSOBIR1 interacts with Cf-4 and Ve1, but not with various RLKs. Tagged versions of Cf-4, Ve1, AtCLV1, SlSERK1, SlSERK3a/BAK1, and SlFLS2 (all fused to eGFP, except for SlFLS2, which was fused to GFP) were coexpressed with SlSOBIR1–Myc in N. benthamiana. Total protein extracts of transiently transformed leaf tissue were subjected to immunopurification by using GFP-affinity beads. Total proteins (Input) and immunopurified proteins (IP) were subjected to SDS/PAGE and blotted. Blots were incubated with α-GFP antibody to detect the immunopurified (e)GFP fusion proteins and incubated with α-Myc antibody to detect coimmunopurifying SOBIR1–Myc proteins. Coomassie-stained blots showing the 50-kDa Rubisco band present in the input samples confirm equal loading. Representative results for three independent experiments are shown.