Fig. 5.

SOBIR1 is required for the accumulation of Cf-4 and Ve1 proteins. Cf-4 and Ve1, fused to eGFP, were expressed in leaves of N. benthamiana subjected to VIGS by inoculation with the indicated TRV constructs. Transiently expressed fusion proteins were immunopurified and subjected to SDS/PAGE, and blots were incubated with αGFP antibody for detection of the expressed proteins. The Coomassie-stained blot shows the 50-kDa Rubisco band present in the input samples to confirm equal loading. The experiment was repeated three times with similar results, and a representative picture is shown.