Fig. 1.

Location of ram mutations in 16S rRNA. (A) Positions of 16S rRNA mutations that increase miscoding (blue), mapped onto the 30S ribosomal subunit (PDB ID code 2WDG), viewed from the subunit interface (Left) and the solvent side (Right). The 16S rRNA, mRNA, and r-proteins are shown as cartoon. The positions of the mutations G299A and G347U (orange), and the head, shoulder, and platform domains and intersubunit bridge B8 of the subunit are indicated. The 30S r-proteins are removed for clarity. (B) Most of the 16S rRNA ram mutations cluster to regions distant from the tRNA binding sites, nearly half of which map to h8/14, which contacts L14 (yellow) and L19 (maroon) to form B8 (boxed). Although mutation G347U is proximal to B8, G299A resides ∼80 Å away.