Fig. 2.

The 16S rRNA mutations G299A and G347U cause similar conformational rearrangements that disrupt intersubunit bridge B8. (A) B8 is formed by contacts between h14 of the 30S subunit and L14/L19 of the 50S subunit (PDB ID code 2WDG), many of which are lost in the mutant structures. Color scheme is the same as in Fig. 1B. (B) 70S G347U (green) and (C) G299A (blue) mutations cause h14 displacement away from L14 and L19, disrupting the hydrogen bonding network between the subunits. The rRNA backbone of the wild-type 70S structure is shown for comparison (gray).