Skip to main content

View full-text article in PMC

. 2013 Apr 29;110(24):9716–9721. doi: 10.1073/pnas.1301585110

Table 1.

Kinetic parameters for EF-Tu–dependent GTP hydrolysis on control and mutant ribosomes

Ribosomes	Cognate		Near cognate		Selectivity^*	Source
Ribosomes	k_GTPmax, s⁻¹	K_1/2, μM	k_GTPmax, s⁻¹	K_1/2, μM	Selectivity^*	Source
Control	51 ± 3	1.1 ± 0.2	1.8 ± 0.1	1.5 ± 0.2	39	This study and ref. 17
G299A	110 ± 9	2.3 ± 0.4	17 ± 1	1.0 ± 0.2	2.7	This study
G347U	130 ± 10	2.8 ± 0.5	15 ± 1	2.5 ± 0.4	7.7	Ref. 17

^*

(Cognate k_GTPmax/K_1/2)/(Near-cognate k_GTPmax/K_1/2).