Table 2.

Products formed from PCP by PcpB

PcpB, µM	PcpD, µM	PCP consumed, µM	TCHQ formed, µM
2.8	0	9.0 ± 1.8	6.0 ± 0.5
2.8	3	13 ± 2.3	10 ± 0.9
2.8	6	14 ± 0.9	17 ± 0.5
2.8	12	20 ± 4.7	24 ± 2.3
2.8	6 (no NADH)	9.0 ± 2.3	5.0 ± 0.1
2.8	10 (PcpD–FeS)*	17 ± 1.6	0
2.8	40 (PcpD-FeS)*	14 ± 5.2	0
5.6	20 (+ 0.5 M potassium phosphate)*	23 ± 5.7	0.4 ± 0.9

Reaction mixtures contained 100 µM PCP, 50 µM NADPH, and 50 µM NADH, except where indicated.

^*Reactions carried out in the presence of 6 mM β-ME.