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. 2013 May 28;110(24):9818–9823. doi: 10.1073/pnas.1302934110

Fig. 5.

Fig. 5.

Injected RNA encoding FLAG-tagged Crygm2d7 is regulated at the translational level by Eif3ha, dependent on UTR sequences. (A) Schematics of C-terminal and N-terminal FLAG-tagged proteins along with the Western blots using anti-FLAG antibodies showing protein levels generated in the control, eif3ha, or eif3c morphant embryos. The endogenous level of β-actin in each lane served as control. (B) Quantitation of the respective Western blots from A. (C) Total RNA was isolated from each set of embryos and subjected to qPCR analysis, as indicated. (D) Schematics of the constructs containing either the 5′ UTR or the 3′ UTR of crygm2d7 along with the respective control β-actin UTR sequences along with Western blots showing the levels of corresponding FLAG-tagged proteins generated in control, eif3ha, or eif3c MO-injected embryos. The endogenous level of β-actin in each lane, served as control. (E) Quantification of the respective Western blots from D. (F) Total RNA was isolated from each set of embryos and subjected to qPCR analysis, as indicated.