Fig. 1.

Purification of the PSII-LHCII-LHCSR3 supercomplex from WT C. reinhardtii. (A) Thylakoids from WT (137c) cells grown under HL conditions (500 μE/m²/s) were solubilized with α-DM and subjected to SDG centrifugation. The solubilized membranes (200 μg Chl) were loaded on a gradient. The four green bands were identified as dissociated LHCIIs (monomers and trimers), the PSI-LHCI supercomplex, and the PSII-LHCII supercomplex (24). (B) Polypeptides in the SDG fractions shown in A were analyzed by SDS/PAGE and staining with Coomassie brilliant blue R-250. (C) Polypeptides in the SDG fractions from the α-DM–solubilized WT thylakoids were subjected to immunoblotting with an antibody against LHCSR3.