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. 2013 May 28;110(24):10016–10021. doi: 10.1073/pnas.1222606110

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Fig. 2. — Polypeptide composition of affinity-purified photosystems. (A) Immunoblotting analyses with antibodies against each photosynthetic protein were performed as labeled. The PSII and PSI supercomplexes isolated by nickel-affinity chromatography were obtained from PsbH-His and PsaA-His strains, respectively; 1 μg of Chl was loaded in each lane. Thy, thylakoids; Elut, elution fraction; Flow, flow-through fraction. (B) The affinity-purified PSII-LHCII-LHCSR3 supercomplexes were subjected to SDG fractionation and immunologically characterized using the specific antibodies as labeled.