Fig. 1.

TRAPPIII contains seven subunits and is conserved from yeast to man. (A, Left) Trs85–TAP was purified from yeast as described before (30). Purified TAP-tagged Trs85 yields a complex that contains seven major subunits. (Right) Mass spectrometry analysis of purified TAP-tagged Trs85 from yeast. (B) NIH 3T3 lysate was fractionated on a Superdex-200 column as described in Materials and Methods and immunoblotted for mTrs85, mTrs130, and mTrs20. (C) mTrs120 (Left) or mTrs85 (Right) were immunoprecipitated from lysates, and the immunoprecipitates were blotted for the presence of mTrs85, mTrs130, mTrs120, and mTrs20. Similar results were obtained when HeLa or COS-7 cells were used for the precipitations.