Skip to main content
. 2013 May 28;110(24):9974–9979. doi: 10.1073/pnas.1221381110

Fig. 1.

Fig. 1.

Effect of SD on the expression of Nlg1 transcript variants. (A) Scheme of the Nlg1 mRNA showing position of splice sites and qPCR amplicons (A and NA or B and NB, with and without insert A or B, respectively; C, common probe). Dark gray, cholinesterase domain (94–1,878 bp); blue, transmembrane domain (2,086–2,142 bp); light blue, PSD95-binding domain (2,512–2,529 bp). (B) Relative expression of Nlg1 transcripts in the forebrain of C57BL/6J (B6) and AKR/J (AK) mice at ZT6 (6 h after light onset) under the control condition (n = 4 for B6 and AK) or after a 6-h SD (n = 3 for B6, 4 for AK). SD significantly decreased the expression of Nlg1 with insert B and common Nlg1 (condition effects: F1,12 ≥ 5.1, *P < 0.05) and increased the expression of Nlg1 with A only in AK mice (interaction: F1,12 = 6.8, *P < 0.05: compared with Control). AK mice also expressed more common Nlg1 and Nlg1 without A and without B than B6 mice (strain effects: F1,12 ≥ 9.8, P < 0.01). (C) Expression of Nlg1 transcripts in the forebrain of DBA/2J (D2) mice at ZT6 under the control condition or after a 6-h SD in mice submitted to either a sham surgery (n = 7/group) or an ADX (n = 6/group). SD decreased the expression of Nlg1 without A and with B (condition effects: F1,22 ≥ 4.2, *P ≤ 0.05), and a similar tendency was found for common Nlg1 (F1,22 = 3.7, ∆P < 0.07).