Fig. 3.

KMD proteins physically interact with type-B ARR proteins. (A) Yeast two-hybrid assay between KMD proteins (KMD1 or KMD2) and type-B ARR proteins. Pairs of indicated bait and prey vectors were transformed into yeast cells. The growth of a blue yeast colony on selective medium containing X-gal indicates a positive interaction. (B) Interaction between KMD proteins and ARR1 or ARR12 in BiFC assay. The indicated constructs were cotransfected into the Arabidopsis mesophyll protoplasts. (C) Coimmunoprecipitation of type-B ARR proteins (ARR1 or ARR12) and KMD1. Protoplast cells were cotransfected with ARR1-HA or ARR12-HA along with either KMD1-GFP or a GFP control. The KMD1-GFP or GFP proteins were immunoprecipitated with an anti-GFP antibody, and an immunoblot was probed with anti-HA and anti-GFP antibodies.