Fig. 6.
CCT knockdown inhibits translocation of LFN-Bla to the cytosol of host cells. (A) β-Lactamase activity after J774A.1 cells were incubated with PA and LFN-Bla at 37 °C. β-Lactamase activity was measured by FRET using the substrate CCF2AM. Uncleaved substrate emits green fluorescence, and cleaved substrate emits blue fluorescence. (B) β-Lactamase activity after LFN-Bla was delivered to the cytosol through PA pores in the cell membrane. Data are expressed as mean ± SD of two technical replicates. *P < 0.05.