Fig. 1.

Impaired liver regeneration in mice lacking eosinophils. (A and B) Eosinophil numbers in livers of mice subjected to partial hepatectomy (PH) or toxin-induced injury (CCl₄) were enumerated using flow cytometry. Migration of eosinophils into injured livers is integrin-dependent and can be blocked by antibodies against α₄ and α_L integrins (Ab). Isotype (Iso) immunoglobulins (IgG2a and IgG2b) were administered to control mice (n = 4–12 mice per group). (C) PH was performed in WT and ΔdblGATA mice, which lack eosinophils, and BrdU incorporation was examined at the indicated times to assess hepatocyte proliferation (n = 4–7 mice per genotype per time). (D) Remnant liver sections were stained for BrdU 36 h after partial hepatectomy. Representative images of WT and ΔdblGATA are shown. (E) Percentage of Ki67⁺ hepatocytes in WT and ΔdblGATA mice 2 d after administration of CCl₄ (n = 3–5 mice per genotype). (F) Quantification of necrotic area in WT and ΔdblGATA mice 2 d after administration of CCl₄ (n = 4–5 mice per genotype). *P < 0.05, **P < 0.01, ***P < 0.001. All data are presented as mean ± SEM.