Fig. 2.

Class I HDAC inhibitor-mediated suppression of ERK1/2 phosphorylation requires gene transcription and phosphatase activity. (A) NRVMs were stimulated with PE in the absence or presence of the class I HDAC inhibitor MGCD0103 for 42 h before treatment with DMSO vehicle or actinomycin D (Actino D) for 6 h. (B) NRVMs were treated with PE in the absence or presence of MGCD0103 for 46 h. Cells were further treated with the phosphatase inhibitor, pervanadate (PV; 75 μM), for 2 h before harvesting for immunoblotting with the indicated antibodies.