Glucose and insulin tolerance test in NHA2 KO and genetrap mice. (A) Blood glucose levels measured in whole blood following i.p. glucose challenge (2 g per kg of body weight) in KO mice and heterozygous and WT littermates (n+/+ = 12; n+/− = 10; n−/− = 10). Data are means ± SEM. *P < 0.05, WT vs. KO; #P < 0.05, WT vs. heterozygous. (B) Insulin concentration measured in sera of KO mice and heterozygous and WT littermates following i.p. glucose challenge (2 g per kg of body weight) (n+/+ = 9; n+/− = 10; n−/− = 10). Data are means ± SEM. *P < 0.05, WT vs. KO. (C) Blood glucose levels measured in whole blood following i.p. insulin challenge (1 U per kg of body weight) in KO mice and WT littermates (n+/+ = 12; n−/− = 12). Data are means ± SEM. (D) Blood glucose levels measured in whole blood following i.p. glucose challenge in genetrap NHA2 mice and WT littermates (NHA2gt
−/− and NHA2gt
+/+, respectively; n+/+ = 12; n−/− = 12). Data are means ± SEM. *P < 0.05, WT vs. NHA2-deficient. (E) Insulin concentration measured in sera of genetrap NHA2 mice and WT littermates following i.p. glucose challenge (2 g per kg of body weight) (n+/+ = 12; n−/− = 12). Data are means ± SEM. *P < 0.05, WT vs. NHA2-deficient. (F) Blood glucose levels measured in whole blood following i.p. insulin challenge (1 U per kg of body weight) in genetrap NHA2 mice and WT littermates (n+/+ = 9; n−/− = 11). Data are means ± SEM.