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. 2013 May 29;110(24):10004–10009. doi: 10.1073/pnas.1220009110

Fig. 4.

Fig. 4.

Reduced secretagogue-induced insulin secretion in NHA2-deficient islets. (A) Basal and glucose-induced (20 mM) insulin release by isolated islets of NHA2 KO mice and heterozygous or WT littermates normalized to insulin content (n = 18 per genotype and condition). Data are means ± SEM. *P < 0.05 vs. WT. (B) Tolbutamide-induced (250 µM) insulin release by isolated islets of NHA2 KO mice and heterozygous or WT littermates normalized to insulin content (n = 8 per genotype and condition). Data are means ± SEM. *P < 0.05 vs. WT. (C) KCl-induced (50 mM) insulin release by isolated islets of NHA2 KO mice and heterozygous or WT littermates normalized to insulin content (n = 8 per genotype and condition). Data are means ± SEM. *P < 0.05 vs. WT; NS, not significant. (D) Insulin secretion of perifused islets of NHA2 WT (n = 7) and KO mice (n = 7) by low (2 mM) and high (20 mM) glucose. Data are means ± SEM. *P < 0.05 vs. KO. (E) Islet insulin content (n = 68 per genotype). (F) Islet proinsulin content (n = 24 per genotype). (G) Ratio of insulin vs. proinsulin secretion in 20 mM glucose (n = 12 per genotype). Data are means ± SEM. (H) Effect of 180 µM phloretin or vehicle (DMSO) on tolbutamide-induced (250 µM) insulin secretion in NHA2 KO mice and WT littermates normalized to insulin content (n = 10 per genotype). Data are means ± SEM. *P < 0.05; NS, not significant. (I) Effect of 180 µM phloretin or vehicle (DMSO) on KCl-induced (50 mM) insulin secretion in NHA2 KO mice and WT littermates normalized to insulin content (n = 10 per genotype). Data are means ± SEM. NS, not significant.