Fig. 3.

Altered inflammatory response in the intestine of Clock mutant mice after in vivo infection with S. Typhimurium. (A) Tissue colonization in WT and Clock mutant mice 72 h p.i. with S. Typhimurium. Day, 10:00 AM, ZT4; night, 10:00 PM, ZT16. Each circle represents an individual animal. Red bars indicate the geometric means (n ≥ 8). WT mice are as in Fig. 1A and Fig. S1A. (B) Histopathology of cecum of infected Clock mutant mice from A. Each bar represent the combined score of at least eight mice. PMN, polymorphonuclear leukocytes. (C) Representative images (10× magnification) of the ceca from Clock mutant mice at 72 h p.i. (Salmonella) or mock, at day or night.