Fig. 5.

Inducible expression of ATG7-targeting shRNA inhibits androgen-mediated lipid accumulation and cell growth. A, Diagram of the pINDUCER11 construct used in this study. B and C, To characterize the LNCaP ATG7 shRNA stable cells, cells were treated for 2 d ± 800 ng/ml Dox and subjected to fluorescence microscopy (B) or Western blot analysis (C). D, TG levels were quantitated as described in Fig. 4 but using LNCaP ATG7 shRNA-inducible stable cells cotreated for 3 d ± Dox and ± R1881. Results are expressed as TG levels normalized to cell numbers + se (n = 2). *, Significant changes from double vehicle-treated cells. E, Stable cells were again cotreated but for 7 d followed by quantification of cell numbers using the assay described in Fig. 1. Results are expressed as mean relative cell number + se (n = 2). **, Significant change (P < 0.01) from R1881 alone-treated cells.