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. 2012 Dec 4;27(2):224–237. doi: 10.1210/me.2012-1310

Fig. 1.

Fig. 1.

Attenuation of GnRH-induced β-catenin nuclear accumulation and FSHβ mRNA expression by JNK inhibition. A, Effect of SP600125 on GnRH-induced nuclear accumulation of β-catenin in LβT2 cells. Cells were serum starved overnight, pretreated with 40 μm SP600125 or vehicle for 30 min, and stimulated with 10 nm GnRH or vehicle for 15 min. Nuclear extracts were subjected to a quantitative Western blot analysis using a β-catenin-specific antibody. LSD1 (in red) was used as a loading control. B, Quantification of Western blot densitometry from three independent experiments, plotted as mean ± sem. One-way ANOVA (n = 3; *, P < 0.05). C, Effect of SP600125 on GnRH-induced FSHβ mRNA levels. Cells were serum starved overnight, pretreated with 40 μm SP600125 or vehicle for 30 min, and stimulated with 5 nm GnRH or vehicle for 6 h. Relative mRNA copy numbers of FSHβ were determined by quantitative real time-PCR. Two-way ANOVA (n = 4; **, P < 0.01).