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. 2012 Dec 4;27(2):224–237. doi: 10.1210/me.2012-1310

Fig. 7.

Fig. 7.

Role of Brms1L in β-catenin regulation of GnRH-induced FSHβ expression. A, Knockdown efficiency of Brms1L siRNA in LβT2 cells. LβT2 cells were transfected with either scrambled or Brms1L siRNA for 48 h, serum starved overnight, and stimulated with 5 nm GnRH or vehicle for 2 h. Medium was replaced with fresh culture medium for another 4 h. B, Effect of siRNA-mediated silencing of Brms1L on GnRH-induced FSHβ mRNA levels. Cells were treated as described in panel B. C, Effect of siRNA-mediated silencing of β-catenin on Brms1L mRNA levels. Cells were transfected with either scrambled or β-catenin siRNA for 48 h, serum starved overnight, and stimulated with 5 nm GnRH or vehicle for 2 h. D, Effect of JNK inhibition on Brms1L mRNA levels. Cells were serum starved overnight, pretreated with 40 μm SP600125 or vehicle for 30 min, and stimulated with 5 nm GnRH or vehicle for 6 h. Relative mRNA copy numbers of Brms1L and FSHβ were determined by quantitative real time-PCR. Two-way ANOVA (n = 4; **, P < 0.05). Results represent the mean ± sem of three independent experiments.