Fig. 2.

Enhanced GHR stability in T47D cells with reduced PRLR. A, Cycloheximide treatment. Serum-starved T47D-SCR and T47D-ShPRLR cells were treated for 0–5 h with CHX, as indicated. Detergent extracts were resolved by SDS-PAGE and immunoblotted. Note that all samples were resolved on the same gels but are separated in the figure according to cell type for ease of visualization. B, Densitometric analysis of data from three separate experiments, performed as in A, was used to estimate GHR abundance. For each cell type, the GHR level present in untreated cells was considered as 100%. Data are expressed as mean ± se for each CHX treatment duration relative to untreated cells for each cell type. *, P < 0.05 when comparing T47D-SCR vs. T47D-ShPRLR cells incubated for the same duration in CHX. WB, Western blot.