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. Author manuscript; available in PMC: 2013 Jun 17.
Published in final edited form as: Mucosal Immunol. 2010 Mar 17;3(3):260–269. doi: 10.1038/mi.2010.10

Figure 6.

Figure 6

Gastric HLA-DR+ dendritic cells (DCs) drive a Th1-predominant response. (a) Gastric HLA-DR+ DCs, (b) gastric CD13+ cells or (c) monocyte-derived DCs (MoDCs) derived from cells from healthy subjects were pulsed with H. pylori, multiplicity of infection 20 for 2 h, washed twice, and co-cultured with autologous blood CD4+ T cells for 3 days. Supernatants from the second wash (“wash”) were used as controls to exclude direct effects of residual bacteria on the T cells. Concentrations of interfreron (IFN)-γ and interleukin (IL)-10 in culture supernatants were determined by enzymelinked immunosorbent assay. Data are representative of three (gastric HLA-DR+ DCs) or two (gastric CD13+ cells and MoDCs) experiments with cells from separate subjects; values correspond to mean ± s.e.m.. Insets in the lower panels show the ratio of IFN-γ to IL-10 for two or three experiments (mean ± s.e.m.) at an antigen-presenting cell (APC)/T-cell ratio of 1:10; * P <0.05, unpaired Student’s t -test.