Table 1.
% HLA DR+a |
% CD13+ |
% MoDCb |
|||
---|---|---|---|---|---|
H. pylori status | Non-infected | Infected | Non-infected | Infected | Non-infected |
n | 4 | 3 | 3 | 3 | 4 |
CD11cc | 18.6 ± 1.8%d | 46.6 ± 5.0%*** | 5.2 ± 2.9% | 6.9 ± 2.6% | 96.7 ± 3.0% |
CD14 | 14.4 ± 2.8 | 22.3 ± 2.1 | 4.1 ± 1.6 | 9.8 ± 2.6 | 2.8 ± 0.6 |
CD80 | 2.8 ± 0.6 | 12.8 ± 8.1 | 1.6 ± 0.7 | 0.9 ± 0.4 | 15.2 ± 5.8 |
CD83 | 2.0 ± 0.3 | 6.3 ± 2.6* | 2.3 ± 1.1 | 4.4 ± 1.3 | 5.9 ± 2.1 |
CD86 | 7.5 ± 0.9 | 20.3 ± 3.9** | 2.4 ± 1.5 | 5.2 ± 4.2 | 59.7 ± 11.8 |
CD206 | 14.5 ± 4.3 | 5.8 ± 0.0e | 2.7 ± 2.0 | 0.3 ± 0.0e | 86.2 ± 9.7 |
DC-SIGN | 14.1 ± 6.3 | 25.1 ± 10.1 | 3.1 ± 1.6 | 5.6 ± 1.7 | 91.5 ± 3.9 |
Abbreviation: MoDC, monocyte-derived dendritic cells.
Gastric mucosal cell subsets were enriched by MACS.
Immature MoDCs (day 6) were used as controls.
Surface markers were analyzed by FACS, with cells gated as either HLA DRhigh or CD13+ cells.
Mean ± s.e.m.
n = 1
P<0.001;
P<0.01;
P<0.05