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. Author manuscript; available in PMC: 2013 Jun 17.
Published in final edited form as: Mol Biochem Parasitol. 2007 May 13;155(1):18–25. doi: 10.1016/j.molbiopara.2007.05.003

Table 2.

Microsatellite length polymorphism detected by sequencing P. berghei wild type and PbMSH2-2 disrupted gDNA

Primer set Laboratory strains Differences in mutant and control strains relative to NK65 parent strain
ANKA NK65 MSH2-2 mutant clones Mosquito-passaged parasites
A2a A2b B2 NKm A2c A2d C2
MS7a (ATA)24 (ATA)22 NC NC NC NC NC NC NC
MS8 (ATA)24 (ATA)25 NC NC NC NC NC NC NC
MS11 (TAT)29 (TAT)27 NC NC NC NC NC NC NC
MS13 (TTA)24 (TTA)20 NC NC NC NC NC NC NC
MS18a (TTA)14 (TTA)16 NC NC NC NC NC NC NC
(TAG)6 (TAG)6
MS21 (AAT)13 (AAT)13 NC NC NC NC (AAT)11 (AAT)11 NC
MS23 (ATA)8 (ATA)8 (ATA)9 (ATA)9 (ATA)9 NC (ATA)9 (ATA)9 NC
(ATA)5 (ATA)5 (ATA)5 (ATA)5 (ATA)5 (ATA)5 (ATA)5
CG8 (AAT)22 (AAT)23 NC NC NC NC NC NC NC
(A)13 (A)13
CG9 (TTA)24 (TTA)20 NC NC NC NC NC NC NC
CG11 (TAT)29 (TAT)27 NC NC NC NC NC NC NC
CG12 (ATA)6 (ATA)6 NC NC NC NC NC NC NC
(TAA)5 (TAA)5
(TAA)5 (TAA)5
(TAA)5 (TAA)5
CG13 (ATTT)40 (ATTT)40 NC NC NC NC NC NC NC
CG16 (T)10 (T)10 NC NC NC NC NC NC NC
(AT)5 (AT)5
(TTAT)21 (TTAT)20
CG17 (A)18 (A)18 NC NC NC NC NC NC NC
(GAT)5 (GAT)5
(GAT)5 (GAT)5
(GAT)6 (GAT)6
CG25 (AT)22 (AT)22 NC NC NC NC NC NC NC

A2a–d: populations of MSH2-2 mutant clone A2, isolated from separate rodent infections; B2: MSH2-2 mutant clone B2; NKm: NK65 wild type parasites passaged through the mosquito stages of the life cycle; C2: Map2K mutant control clone; NC: no change

a

Microsatellite region Blasts to putative hypothetical protein in the PlasmoDB database