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Schematic of three-step SpuTR enrichment from total RNA. TMG-capped (red) RNA was purified from total RNA by anti-TMG IP and was then treated with 5′-monophosphate-specific exonuclease (orange) to remove ribosomal RNA. Recombinant SBP-tagged SpuTERT (blue) synthesized in vitro was affinity purified from RRL by streptavidin Sepharose beads and incubated with the TMG-capped exonuclease-treated RNA.
