Computational screening for SpuTR candidates. Sequencing reads (9,051,612) were mapped onto the purple sea urchin reference genome. Mapped loci (181,881) were screened for putative template sequences, resulting in 1173 putative template loci. Putative template loci matching known or hypothetical genes were discarded, resulting in 654 candidate loci. Inputs (white rounded boxes), processes (gray diamonds), and final output (black square) are depicted.