FIGURE 5.

Efficacy of individual TR enrichment steps. Total RNA and RNA samples from each of the three sequential TR enrichment steps (anti-TMG IP, exonuclease-treatment, and TERT-binding) were analyzed by Illumina next-generation (Next-Gen) sequencing. Read coverage for SpuTR in each sample and fold enrichment of coverage between samples (curved arrows) are indicated. Ranking the SpuTR locus and the number of template-containing loci were determined by bioinformatics screening and shown for each sample. Sequencing reads corresponding to the SpuTERT expression vector contaminated from the RRL reactions were removed prior to bioinformatics screening.