TABLE 2.

Values of affinity constants for pyruvate and NADH in the presence of 10 mM FBP (R state) or in the presence of 50 mM P_i (T state) as obtained in the standard assay^a

Strain	KR,Pyr (mM)	KR,NADH (mM)	VR,MAX (U/mg)	KT,PYR (mM)	KT,NADH (mM)
65.1	1.4	0.03	11.5	18	0.13
MG 1363	1.1	0.03	9.3	2.0	0.15
NCIMB701197	1.2b	0.04b	9.3b	7.7b	0.40b
NCIMB700509	1.4c	0.05c	13c	4.9c	0.14c
NCIMB700763	0.4	0.10	10.2	3.4	0.20
IL 1403	1.1	0.06	11.6	4.1	0.14
ATCC 19435	2.8	0.03	11.0	6.6	0.12
NCDO 2118	2.4	0.05	4.5	4.4	0.15

^aK_R,Pyr and K_R,NADH, affinity constants for pyruvate and NADH, respectively, in the R state; K_T,Pyr and K_T,NADH, affinity constants for pyruvate and NADH, respectively, in the T state.

^bTaken from literature (9).

^cTaken from literature (3).