TABLE 3.

Variables used in V_LDH = V_R,max · [α_Pα_NRⁿ⁻¹/(Rⁿ+LTⁿ)] · (1 − δ) · (1 − ɛ − η), an equation for the expression of lactococcal LDH^a

Variable	Equivalency
αP	[pyruvate]/KR,Pyr
cP	KR,Pyr/KT,Pyr
δ	[NAD]/NADcrit
η	[ADP]/ADPcrit
β	[FBP]/KFBP,0.5V
R	1 + αP + αN + αPαN
αN	[NADH]/KR,NADH
cN	KR,NADH/KT,NADH
ɛ	[ATP]/ATPcrit
L	L0 · [(1 + γ)/(1 + β)]m
γ	[Pi]/KP,0.5V
T	1 + cPαP + cNαN + cPαPcNαN

^aV_R,max, maximum enzyme rate in the R state; L, the equilibrium between the T and R states; K_R,Pyr and K_T,Pyr, affinity constants for pyruvate in the R and T states, respectively; NAD_crit, ADP_crit, and ATP_crit, concentrations of NAD, ADP, and ATP, respectively, at which LDH is expected to be inhibited completely based on the equation of Han and Levenspiel (6); K_FBP,0.5V, FBP concentration at which LDH was 50% activated in the absence of P_i; K_R,NADH and K_T,NADH, affinity constants for NADH in the R and T states, respectively; L₀, equilibrium between the T state and the R state of the LDH enzyme without substrate; K_P,0.5V, phosphate concentration at which LDH was 50% inhibited, as extrapolated to 0 mM FBP; m and n, number of modulator sites.