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. 2004 Mar;70(3):1413–1424. doi: 10.1128/AEM.70.3.1413-1424.2004

FIG. 4.

FIG. 4.

Detection and identification of Gigasporaceae from DNA extracted from 2-month-old clover roots by PCR-DGGE analysis of the V9 region of the 18S rRNA gene. DNA templates: lane 1, S. heterogama CNPAB2; lane 2, G. margarita CNPAB16; lane 3, Trifolium pratense replicate 1; lane 4, T. pratense replicate 2; lane 5, T. pratense replicate 3; lane 6, S. gregaria CNPAB7; lane 7, S. reticulata CNPAB11. Note the presence of secondary bands in lanes 4 and 5 (S. heterogama) and 3 (S. reticulata) (arrows). The gel was run for 17 h at 95 V.