TABLE 3.

rDNA primers, primer combinations, GC clamp, and PCR conditions used in this study^a

Primer	Sequence	Target group	Partner primer for PCR	PCR conditions	Product size (bp)
NS1	5′-GTAGTCATATGCTTGTCTC-3′	Universal eukaryotes	ITS4	94°C for 60 s, 55°C for 240 s, 30 cycles	2,300
NS31-GC	5′-TTGGAGGGCAAGTCTGGTGCC-3′	Universal eukaryotes	AM1	94°C for 60 s, 61°C for 40 s, 30 cycles	600
FM6	5′-ACCTGCTAAATAGTCAGGCTA-3′	Gigasporaceae	GIGA5.8R	94°C for 60 s, 59°C for 45 s, 30 cycles	700
NS7-GC	5′-GAGGCAATAACAGGTCTGTGATGC-3′	Universal eukaryotes	F1Ra	94°C for 60 s, 60°C for 28 s, 30 cycles	400
ITS4	5′-TCCTCCGCTTATTGATATGC-3′	Universal eukaryotes
AM1	5′-GTTTCCCGTAAGGCGCCGAA-3′	Fungi
GIGA5.8R	5′-ACTGACCCTCAAGCAKGTG-3′	Gigasporaceae
F1Ra	5′-CTTTTACTTCCTCTAAATGACC-3′	Fungi

^aPrimer sources: NS1, NS7, and ITS4, White et al. (57); NS31, Simon et al. (49); AM1, Helgason et al. (23); GIGA5.8R, Redecker (39); FM6 and F1Ra, this study. The GC clamp (5′-CGC CCG GGG CGC GCC CCG GGC GGG GCG GGG GCA CGG GGG-3′) was attached to the 5′ end of primers NS31 and NS7.