Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Purpose of PRL-1 Mutants
| PRL-1 Variant | Purpose |
|---|---|
| Wild Type (WT) | Permits chemical modification of the disulfide bond |
| C104S | Disrupts the disulfide bond directly; abolishes enzymatic activity |
| C49S | Disrupts the disulfide bond directly; retains activity |
| Y53E | Breaks hydrogen bond between Y53 and H103; increases solvent exposure of loop 3 (L3) |
| Y53F | Breaks hydrogen bond between Y53 and H103; retains apolar side chain |
