Table 2.

Physical Stability of PRL-1^a

	Phosphate				HEPES
	CD		SLS		CD		SLS
	Tm	± SD	Onset T	± SD	Tm	± SD	Onset T	± SD
oxWT	65.47	1.11	59.92	0.28	60.54	2.08	53.27	0.35
redWTbc	67.81	0.43	63.75	0.38	61.98	1.26	57.35	0.32
redWTd	79.01e	0.66	ndf		nd		nd
C49S	62.79	2.00	59.68	0.22	56.25	0.32	49.95	0.32
C104S	76.31	1.07	72.1	0.32	64.85	2.06	59.28	0.22
Y53E	62.25	0.35	nd		nd		nd
Y53F	62.61	0.30	nd		nd		nd

^aAll temperatures reported are in °C

^bFor CD, reducing conditions were achieved by the addition of 1 mM DTT. For SLS, reducing conditions were achieved by the addition of 10 mM DTT.

^cCD and/or SLS were also used to study the physical stability of PRL-1 mutants under both reducing and nonreducing conditions, although no differences were detected. This data for this is not shown.

^dReduced PRL-1-WT (redWT) was studied in 20 mM phosphate buffer at pH 6.5 with equivalent ionic strength (I=100 mM). All other values reported, including those for HEPES buffer, are at pH 7.5.

^eMelting was incomplete at the maximum temperature attainable and the data was extrapolated to determine the T_m.

^fNot determined