Skip to main content
. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Nat Methods. 2013 May 5;10(6):584–590. doi: 10.1038/nmeth.2472

Figure 2. Motion correction restores the lost high-resolution information.

Figure 2

a. Fourier transforms of an image of frozen hydrated archaeal 20S proteasomes. This is a near “perfect” image where Thon rings extend to near 3 Å. The cross correlation (CC) between image Thon rings at 10 ~ 5Å and simulated ideal Thon rings over the same resolution range is 0.192. b. The 24 individual subframes were cross correlated and relative positional shifts determined as described in the text and Supplementary Methods. Based on these calculations, the path of motion between the first subframe (large black dot) and last can be determined. c. Fourier transform of the same image after motion correction, where the Thon ring CC is now 0.233. d. Fourier transform of a worse image showing a predominantly unidirectional resolution cut-off at ~20Å. The Thon ring CC is only 0.092. e. The trace of motion between subframes. f. Fourier transform of the same image after motion correction showing that resolution has been isotropically restored, and the Thon ring CC improved to 0.238. The narrow white band was caused by residual fixed pattern noise in each frame, which was subsequently eliminated.