Skip to main content
. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Nat Methods. 2013 May 5;10(6):584–590. doi: 10.1038/nmeth.2472

Figure 4. Subregion motion correction.

Figure 4

a. An image of vitreous ice embedded 20S proteasomes taken completely within a single hole. Red and blue squares represent two different subregions of 2048 × 2048 pixels. b. Comparison of displacement traces determined from the two subregions (black and blue) with that determined using the entire subframe (red). Note that the trends of motions in the subregions are similar, although their speeds in the first few subframes are different. c. Subframe displacement traces from the same image after removing the first 5 frames. The difference between two regions is significantly less than that in b. d–f. Similar to a–c, but the image spans both the hole and the surrounding supporting carbon film. The displacement traces from subregions of carbon film and vitreous ice indicate that they behave very differently under the electron beam. After removal of the first 5 subframes, the remaining shifts are similarly reduced.