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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Nat Methods. 2013 May 5;10(6):584–590. doi: 10.1038/nmeth.2472

Figure 5.

Figure 5

Final 3D reconstruction of archaeal 20S proteasome reveals clear side chain detail

a. 3D density map of T. acidophilum 20S proteasome filtered to a resolution of 3.3 Å. b. Two different views of asymmetrical α- and β-subunit segmented from the 3D density map shown in a. The main chain can be traced throughout the entire map. c. Two α-helices segmented from the α-and β-subunits showing clear density for the majority of side chains. d. A portion of the cryoEM density map showing clear side chain densities. The docked atomic structure was refined to fit the density map by molecular dynamic flexible fitting procedure. e. The same portion of a 2Fo-Fc map of 3.4 Å crystal structure calculated using the atomic structure (pdb code: 1PMA).