FIG. 2.
(a) SDS-PAGE pattern of SDS extracts of whole cells of E. coli HMS174(DE3) containing pET28a carrying the chimeric gene encoding rSbpA31-1068/ZZ before (lane 1) and 1 (lane 2) and 4 (lane 3) h after induction of expression by the addition of IPTG. Lane 4, SDS-PAGE analysis of rSbpA31-1068/ZZ after purification by GPC. Lanes 5 and 6, immunoblot analysis of SDS extracts of purified rSbpA31-1068/ZZ, using polyclonal rabbit antiserum raised against the S-layer protein of B. sphaericus CCM 2177 (lane 5) or antihuman-ALP for detection of bound human IgG (lane 6). (b) Dot blot assays indicating the accessibility of the fused ZZ-domains to human IgG of the water-soluble (S) or the recrystallized S-layer fusion protein (RP). Water-soluble rSbpA was used as a blank (B).