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. Author manuscript; available in PMC: 2013 Jun 17.
Published in final edited form as: Immunol Res. 2011 Apr;49(0):56–69. doi: 10.1007/s12026-010-8194-6

Figure 3.

Figure 3

(A) CD3+ T cell expansion is superior in the presence of IL-7. Frozen/thawed cord blood T cells were enriched by negative selection then split equally into two under identical culture conditions except for the presence of IL-7 as indicated. cell were cultured for 12—14 days with ClinExVivo™ Dynabeads® while medium and cytokines were replenished x 3/week. A 50ul aliquot was removed from the bags at indicated time points and absolute T cells number was enumerated in Trucount® tubes. (B) Irrespective of IL-7 in the culture medium, expansion leads to dilution and near complete loss of sjTREC in day 14 progeny. The signal joint TCR excision circles (sjTREC) were measured before and after expansion, n=4.. For each sample total nucleated cell count and absolute T cells content was enumerated by Trucount FACS method. TREC content was expressed after adjustment for 105 T cells/sample. Reproduced with permission from Canceer Research ©2010,