TABLE 1.
TRF sizes of the 16S rRNA genes from pure actinobacterial species digested with HinfI, HhaI, and MboI
| Name | Species | Enzyme | Expected fragment size (bp) | Actual fragment size (bp) |
|---|---|---|---|---|
| EN 45 | Streptomyces galilaeus | HinfI | NAa | 240.6 |
| HhaI | NA | 419.2 | ||
| MboI | NA | 158.1 | ||
| AB6 | Streptomyces bottropensis | HinfI | 236 | 238.6 |
| HhaI | 419 | 418.5 | ||
| MboI | 158 | 158.1 | ||
| PM 23 | Streptomyces caviscabies/setonii | HinfI | NA | 180.0 |
| HhaI | NA | |||
| MboI | NA | 158.1 | ||
| 162.4 | ||||
| S. scabies | Streptomyces scabies (ATCC 49173) | HinfI | 236 | 239.6 |
| HhaI | 418 | 415.7 | ||
| MboI | 157 | 157.3 | ||
| SE2 | Streptomyces caviscabies/setonii | HinfI | NA | 238.5 |
| HhaI | NA | 419.0 | ||
| MboI | NA | 158.0 | ||
| S. setonii | Streptomyces setonii (ATCC 25497) | HinfI | 236 | 239.9 |
| HhaI | 419 | |||
| MboI | 158 | 158.3 |
a
NA, no entry found in the RDP database by using the TAP T-RFLP software.