Table 2.

Techniques applied to skin biopsies with their relevant applications

Technique	Applications	References
Immunohistochemistry	IENF density	Kennedy et al. [85]
	Pilomotor innervation	Nolano et al. [20•]
	Sudomotor innervation	Gibbons et al. [18]
	Internodal length	Li et al. [86]
	Nodal width	Nolano et al. [23], Provitera et al. [87]
	Myelin G ratio	Nolano et al. [23]
	Axon diameter	Nolano et al. [23], Provitera et al. [87]
Immuno-EM	Percent protein expression	Katona et al. [88]
EM	Myelin periodicity	Li et al. [86]
	Myelin decompaction	Ceuterick-deGroote et al. [56]
	Localization of mitochondria	Saporta et al. [10]
	Tracking of IENF regeneration	Polydefkis et al. [71]
	Organelle accumulation in axonal swellings	Ebenezer et al. [11]
PCR	mRNA expression	Li et al. [86]

EM electron microscopy, IENF intraepidermal nerve fiber, mRNA messenger RNA